ANALYSIS OF GENETIC POLYMORPHISMS IN ESTROGENMETABOLIZING ENZYMES IN OVARIAN CANCER PATIENTS IN THE SIBERIA REGION

Early diagnosis of ovarian cancer (OC) remains to be the actual problem in modern oncology due to the high mortality rate, asymptomatic
course of the disease in the early stages and its aggressiveness. The aim was to study the genetic polymorphism of pathogenetically significant estrogen
metabolizing enzymes to form risk groups for developing ovarian cancer.
With the use of PCR-RFLP method genetic polymorphism estrogen metabolizing enzymes metabolism (CYP1A1, CYP1A2, CYP19 and
SULT1A1) was analyzed in 96 women with ovarian cancer. As a comparison group 182 women without clinical signs of oncogynecological pathology
were selected. Females with the allele C, genotype A/C and C/C (ApaI polymorphism) of the gene CYP1A2 (OR=2,9, р<0,001; OR=3,4, р<0,001
and OR=16,4, р<0,001, respectively), T allele and genotype C/T (SfaNI polymorphism) gene CYP19 (OR=1,98, р=0,043 and OR=3,9, р=0,001,
respectively), G allele and genotype G / G (HhaI polymorphism) of the gene SULT1A1 (OR=1,5, р=0,02 and OR=1,9, р=0,04, respectively) were
shown to be at increased risk of developing ovarian cancer. For the gene CYP1A1 (MspI polymorphism) no such associations were found. Thus, the
associations of polymorphic variants of the genes CYP1A2, CYP19 and SULT1A1 with hormone-dependent tumors of the ovaries in females have
been identified. The data obtained can be used in the formation of groups at risk of this disease for early diagnosis of ovarian cancer.

N.A. Afanasyeva (2,3), E.P. Khvostova (1), V.O. Pustyl’nyak (1,3), O.B. Chasovnikova (1), S.E. Krasil’nikov (2,3), L.F. Gulyaeva (1,3)
1 -Federal State Budgetary Institution «Research Institute for Molecular Biology and Biophysics of the Siberian Branch of the Russian Academy of Medical Sciences», Novosibirsk, Russian Federation; 2 -Federal State Budgetary Institution of Healthcare «Novosibirsk Regional Cancer Center» of the Novosibirsk region, Novosibirsk, Russian Federation; 3 -Federal State budgetary Institution of Higher professional education «Novosibirsk National Research State University», Novosibirsk, Russian Federation