Abstract
Introduction. Based on current literature data, it is possible to assume that when exposed to glioma cells astrocytes, may undergo a reactive transformation. These glioma-conditioned astrocytes might create a permissive environment for tumorigenesis. The aim of the study. To investigate the effects of glioma cells on astrocytes in co-culture. Methods. In terms of the current work, we conducted a pairwise comparison of protein levels between C6 glioma cells, native rat astrocytes, and glioma-conditioned rat astrocytes. The samples were prepared and analyzed with liquid chromatography–high-resolution mass spectrometry. Results. The analysis showed a significant difference in 162 proteins between glioma cells and native astrocytes, in 141 proteins between glioma cells and glioma-conditioned astrocytes and 70 proteins between glioma-conditioned and native astrocytes. Conclusion. The differences in protein levels between native and glioma-conditioned astrocytes show a high correlation with differences between glioma cells and native astrocytes.
Authors and affiliations
A.S. Silantyev(1), I.V. Chekhonin(2), A.A. Chernysheva(2), O.I. Gurina(2), S.A. Pavlova(3), G.V. Pavlova(3, 4, 5), T.A. Savelieva(6, 7), V.B. Loshhenov(6, 7), V.P. Chekhonin(2, 8) 1-National Scientific Research Center on Addictions a branch of the V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Maly Mogiltsevsky by-street, 3, Moscow, 119002, Russian Federation; 2V-.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Kropotkinskiy by-street, 23, Moscow, 119034, Russian Federation; 3-Institute of Gene Biology Russian Academy of Sciences, Vavilova Street, 34/5, Moscow, 119334, Russian Federation; 4-Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Trubetskaya Street, 8, Moscow, 119991, Russian Federation; 5-N.N. Burdenko National Medical Research Center of Neurosurgery, 4 Tverskaya-Yamskaya str., 16, Moscow, 125047, Russian Federation; 6-Prokhorov General Physics Institute, Vavilova str., 38, Moscow, 119991, Russian Federation; 7-National Research Nuclear University, Moscow Engineering Physics Institute, Kashirskoe Highway, 31, Moscow, 115409, Russian Federation; 8-Pirogov Russian National Research Medical University, Ostrovitianova str., 1, Moscow, 117997, Russian Federation E-mail: aachernysheva512@gmail.com
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