Abstract
Introduction. The study of the molecular mechanisms of skin aging is one of the key problems of dermatocosmetology. Inflamaging is a chronic low–level inflammation that occurs with age. This condition is characterized by a change in the expression of proteins involved in the processes of aging and skin regeneration. Hyaluronic acid preparations containing metals have shown their geroprotective effect in the conditions of inflamaging.
The aim of the studyto identify key biomarkers of cell aging (the development of inflamaging), as well as to study the effect of a hyaluronic acid-based drug with the presence of magnesium in chelated form (Magniderm-09) on human skin fibroblasts in an inflamaging model to assess its possible geroprotective effect.
Material and methods. The study was performed on a culture of skin fibroblasts in a model of inflamaging induced by genotoxic stress. To assess the expression of molecular markers, immunohistochemical analysis of levels of Ki-67, collagen I, III and IV, LOX, ubiquitin, CCN1, IL-8, MMP-3, NF-kB, SIRT1, CD44 was performed.
Results. The modeling of inflamaging revealed a decrease in the expression of Ki-67, all types of collagen, LOX, CCN1, SIRT1, CD44, as well as an increase in proinflammatory cytokines – IL-8, NF-kB, MMP-3 and ubiquitin. Administration of the drug "Magniderm-09" returned expression levels to normal values, which indicates its geroprotective effect.
Conclusion. A correlation has been revealed between the chemical composition of a hyaluronic acid-based hydrogel preparation with the presence of magnesium in chelated form and the molecular biological changes accompanying the process of cellular aging.
Authors and affiliations
Yu.I. Belova(1), E.S. Mironova(1, 2, 4), T.S. Zubareva(1, 2), A.O. Drobintseva(1, 3), D.I. Znatdinov(5)
1-Federal State Budgetary Institution “St. Petersburg Scientific Research Institute of Phthisiopulmonology” of the Ministry
of Health of the Russian Federation, 2–4 Ligovsky Ave., Saint Petersburg, 191036, Russian Federation;
2-ANO SIC “St. Petersburg Institute of Bioregulation and Gerontology”, Russian Federation, 197110, St. Petersburg, Dynamo ave., 3;
3-St. Petersburg State Pediatric Medical University of the Ministry of Health of the Russian Federation,
Russian Federation, 194100, St. Petersburg, Litovskaya str., 2;
4-Saint Petersburg State University, Universitetskaya nab. 7/9, Saint Petersburg, 199034, Russian Federation;
5-ANO “Scientific Research Center of Hyaluronic Acid”, Komsomolsky Ave., 38/16, Moscow, 119146, Russian Federation
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