Abstract
Introduction. Colorectal cancer (CRC) often metastasizes even if the tumor is small in size that is the main cause of death. Complex process of epithelial-mesenchymal transition (EMT) is required for dissemination of tumor cells. EMT leads to the transformation of epithelial phenotype of cells to their mesenchymal phenotype. The aim of the study was the determination of epithelial-mesenchymal transition based on the gene expression profile and somatic molecular genetic alterations in colon cancer samples at different stages, including the peritoneal carcinomatosis. Methods. Forty six CRC patients including cases with peritoneal carcinomatosis were investigated. To determine the program for EMT ZEB1, ZEB2, CDH1, VIM, SNAIL1 gene expression profiling was analyzed with the use of real-time PCR. Somatic mutations in KRAS and BRAF were analyzed by direct sequences. Microsatellite instability was determined by the fragment analysis. Results. Epithelial-mesenchymal transition was detected in 16 cases (34,8%), including 10 of 11 patients with peritoneal carcinomatosis. These mesenchymal subtype tumors were distinguished with high frequency of somatic mutations, microsatellite stability and low grade of differentiation Conclusion. Identification of EMT can provide an indication of high metastatic potential of the tumor that is especially important at the early stages of the disease.
Keywords
Authors and affiliations
N.I. Pospekhova (1), V.P. Shubin (1), A.S. Tsukanov 1), V.N. Kashnikov (1), S.A. Frolov 1), S.I. Achkasov (1), O.I. Sushkov (1), Yu.A. Shelygin (1,2) 1 -State Scientific Center of Coloproctology, Salyama Adilya Str., 2, Moscow, Russian Federation, 123423 2 -Department of Coloproctology, Russian Medical Academy of Postgraduate Education, Salyama Adilya Str., 2, Moscow, Russian Federation, 123423
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