Abstract
Introduction. Oxidative stress and mitochondrial dysfunction are observed in virtually any disease associated with premature aging. Signaling molecules produced by mitochondria can act as specific targets for the action of pharmacological agents in order to regulate the processes of cellular aging, which opens up new possibilities for the search and development of drugs for effective pharmacotherapy of age-associated pathologies conditions.
The aim of the study. To study the effect of V007 on the expression of mitochondrial biomarkers in rat liver cells in vivo to elucidate the possible mechanism of its geroprotective action.
Methods. Molecular biological methods were used to study the expression of key mitochondrial proteins: Tom70, Tom20, VDAC, DRP1, prohibitin, Parkin, PINK1 in liver cells of young and old rats in normal conditions and with the use of the innovative drug V007.
Results. The data obtained indicate that when using the V007 preparation, there is a statistically significant increase in the expression of all studied mitochondrial proteins in hepatocytes of old rats to the level of young animals: DRP1 by 1.3 times; Parkin 1.2 times; PINK1 1.2 times; Prohibitin 1.4 times; Tom 20 1.3 times; Tom 70 1.2 times; VDAC 1.2 times. This change in the expression of mitochondrial proteins is due to the activation of their synthesis and increased activity of the mitochondrial transport systems.
Conclusion. V007 has a general regulatory and geroprotective effect, being a targeted pharmacological agent that regulates the functions of hepatocytes during aging by normalizing the expression of key mitochondrial proteins.
Authors and affiliations
I.M. Kvetnoy(1, 2), E.S. Mironova(1, 2), Y.S. Krylova(2), A.A. Mylnikova(2), T.S.
Zubareva(2), D.O. Leont’eva(2), V.O. Polyakova(2), M.A. Petrosyan(2),
1-Iva Pharm LLC, Pskovskaya st., 17, St. Petersburg, 190121, Russian Federation;
2-St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation,
Ligovsky prospect, 2–4, St. Petersburg, 191036, Russia