Abstract
Introduction. Thyroid disease is currently the most common among endocrine pathology. Hyperproduction of thyroid hormones is accompanied by stimulation of humoral reactions, contributing to the development of immune-mediated pathology under certain conditions, which actualizes the need to develop means for correcting neuroimmunoendocrine processes. Gliproline compounds analogous to neuropeptide protein molecules, among which are Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro, can be considered as such agents.
The aim of the study. The aim of the study was to study the immunotropic properties of gliproline compounds Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro under experimental hyperthyroidism.
Material and methods. The immunotropic properties of the gliproline compounds Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro were studied in white nonlinear male rats. Hyperthyroid status in animals was modeled by intragastric administration of L-thyroxine sodium salt pentahydrate («Sigma»; USA) at 150 μg/kg for 21 days. Laboratory animals were divided into groups (n=10): control group – intact animals (control); animals treated with L-thyroxine sodium salt pentahydrate (hyperthyroidism); rats receiving Thr-Lys-Pro-Arg-Pro-Gly-Pro (selanc) and rats receiving Pro-Gly-Pro intraperitoneally daily for 21 days at doses of 87 and 33 μg/kg/day.
Results. The obtained results indicate the presence in gliproline compounds Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro of immune correcting activity manifested under the conditions of experimental hyperthyroidism, with the greatest impact on the humoral unit of immunity, which is confirmed by the presence of statistically significant changes in the cellular parameters of the spleen and titer antierythrocytic antibodies in a direct hemagglutination reaction.
Conclusion. Thus, the study of the immunotropic properties of gliproline peptide compounds under experimental hyperthyroidism established the presence of immune-correcting activity in Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro substances.
Keywords
Authors and affiliations
M.U. Sergalieva(1), A.A. Tsibizova(1), V.Kh. Murtalieva(1), L.A. Andreeva(2), N.F. Myasoedov(2), M.A. Samotrueva(1),
1-Astrakhan State Medical University, Bakinskaya St., 121, Astrakhan, 414000, Russian Federation;
2-Institute of Molecular Genetics of the SIC «Kurchatov Institute», akad. Kurchatov Square, 2, Moscow, 123182, Russian Federation
References
Tsai K., Leung A.M. Subclinical hypothyroidism: a review of the clinical literature. Endocrine Practice. 2021; 27 (3): 254–60. DOI: 10.1016/j.eprac.2021.02.002
Taylor P.N., Albrecht D., Schultz A., Gutierrez-Boey G., Lazarus J.H., Dayan K.M., Cosime O.E. Global epidemiology of hyperthyroidism and hypothyroidism. Nature Reviews Endocrinology. 2018; 14 (5): 301–16. DOI: 10.1038/nrendo.2018.18
Nijiela M. Hyperthyroidism in adolescents. Endocrine Compounds. 2021; 10 (11): 279. DOI: 10.1530/EC-21-0191
Ordookhani A., Burman K.D. Hemostasis in overt and subclinical hyperthyroidism. International J. of Endocrinology and Metabolism. 2017; 15 (3): e44157. DOI: 10.5812/ijem.44157
Montesinos M.D.M., Pellizas C.G. The effect of thyroid hormones on innate immunity. Frontiers in Endocrinology. 2019; 10: 350. DOI: 10.3389/fendo.2019.00350
Antonelli A., Fellahi Р., Elia G., Ragusa F., In Pairs S.R., Ruffilli I., Ferrari S.M. Basedova disease: clinical manifestations, immune pathogenesis (cytokines and chemokines) and therapy. Best Practice and Research Clinical Endocrinology and Metabolism. 2020; 34 (1): 101388. DOI: 10.1016/j.beem.2020.101388
Fang S., Lu Y., Huang Y., Zhou H., Fan X. Mechanisms underlying T-cell immunity in Graves’ orbitopathy. Frontiers of Endocrinology. 2021; 12: 648732. DOI: 10.3389/fendo.2021.648732
Marinò M., Latrofa F., Menconi F., Chiovato L., Vitti P. The role of genetic and non-genetic factors in the etiology of basedova disease. Journal of Endocrinological Research. 2015; 38: 283–94. DOI: 10.1007/s40618-014-0214-2
Ferrari S.M., Fallahi P., Ruffilli I., Elia G., Ragusa F., Benvenga S., Antonelli A. Association of other autoimmune diseases in patients with basal disease (with or without ophthalmopathy): a literature review and a large series report. Reviews of autoimmunity, 2019; 18 (3): 287–92. DOI: 10.1016/j.autrev.2018.10.001
Ludwig R.J., Vanhoorelbe&e K., Leypoldt F., Kaya Z., Bieber K., McLachlan S.M., Komorowski L., Luo J., Cabral-Marques O., Hammers C.M., Lindstrom J.M., Lamprecht P., Fischer A., Riemekasten G., Tersteeg C., Sondermann P., Rapoport B., Wandinger K.P., Probst C., El Beidaq A., Schmidt E., Verkman A., Manz R.A., Nimmerjahn F. Mechanisms of Autoantibody-Induced Pathology. Front. Immunol. 2017; 8: 603. DOI: 10.3389/fimmu.2017.00603
Ясенявская А.Л., Самотруева М.А., Цибизова А.А., Мясоедов Н.Ф., Андреева Л.А. Влияние глипролинов на перекисное окисление липидов в гипоталамической и префронтальной областях головного мозга в условиях «социального» стресса. Астраханский медицинский журнал. 2020; 15 (3): 79–85. DOI: 10.17021/2020.15.3.79.85. [Yasenyavskaya A.L., Samotrueva M.A., Tsibizova A.A., Myasoedov N.F., Andreeva L.A. The effect of glyprolines on lipid peroxidation in hypothalamic and prefrontal regions of the brain under conditions of «social» stress. Astrakhanskiy meditsinskiy zhurnal. 2020; 15 (3): 79–85 (in Russian). DOI: 10.17021/2020.15.3.79.85]
Сергалиева М.У., Цибизова А.А., Абдулкадырова Э.И., Андреева Л.А., Башкина О.А., Самотруева М.А., Мясоедов Н.Ф. Влияние селанка и Pro-Gly-Pro на поведенческие реакции белых крыс в тесте «Порсолт» в условиях экспериментального гипертиреоза. Астраханский медицинский журнал. 2021; 16 (2): 53–61. DOI: 10.17021/2021.16.2.53.61. [Sergalieva M.U., Tsibizova A.A., Abdulkadyrova E.I., Andreeva L.A., Bashkina O.A., Samotrueva M.A., Myasoedov N.F. Effect of Selank and Pro-Gly-Pro on behavioural reactions of white rats in the test «Porsolt» in conditions of experimental hyperthyroidism. Astrakhanskiy meditsinskiy zhurnal. 2021; 16(2): 53–61 (in Russian). DOI: 10.17021/2021.16.2.53.61]
Трошина Е.А., Сенюшкина Е.С. Вклад центральных регуляторов иммунного ответа в развитие заболеваний щитовидной железы. Проблемы Эндокринологии. 2019; 65 (6): 458–65. DOI: 10.14341/probl10304 [Troshina E.A., Senyushkina E.S. The value of central regulators of the immune response in the development of autoimmune thyroid diseases. Problemy Endokrinologii. 2019; 65 (6): 458–65 (in Russian). DOI: 10.14341/probl10304]
Ченчак В.А. Особенности действия тироксина на иммунную систему. Международный студенческий научный вестник. 2017; 3. URL: https://eduherald.ru/ru/article/view?id=17190 (дата обращения: 23.07.2023). [Chenchak V.A. Features of the action of thyroxine on the immune system. Mezhdunarodnyy studencheskiy nauchnyy vestnik. 2017; 3. URL: https://eduherald.ru/ru/article/view?id=17190 (date of address: 23.07.2023) (in Russian)]
De Luca R., Davis J., Lin H.Y., Gionfra F., Percario Z.A., Fabrice E., Incerpi S. The interaction of thyroid hormones with the immune response, inflammation and syndrome unrelated to thyroid diseases. Boundaries of Cell Biology and Developmental Biology. 2021; 8: 614030. DOI: 10.3389/fcell.2020.614030