Abstract
Relevance. The search for new antitumor drugs and their selective delivery directly to the tumor site is an important task of modern oncology. For these purposes, currently, the use of various nanoparticles as carriers of medicinal substances is of great importance. The pathomorphological features of tumor cells under the action of salinomycin and nanodiamonds have not been studied enough.
The aim of the work was to study the pathomorphological features of the tumor in mice with transplanted Lewis lung carcinoma, who were treated with the ionoform antibiotic salinomycin and a combination of salinomycin with nanodiamonds.
Material and methods. 20 mice were divided into 4 groups. 1 – control group; 2 – mice received salinomycin; 3 – salinomycin and nanodiamonds; 4 – nanodiamonds. A morphometric study of histological and immunohistochemical tumor preparations stained for PCNA was carried out.
Results. Salinomycin is established to have an antineoplastic action. The use of nanodiamonds did not significantly affect the morphofunctional characteristics of Lewis lung carcinoma and did not change the antitumor activity of salinomycin.
Conclusion. Salinomycin has an antitumor effect and requires further research.
Authors and affiliations
V.V. Popuchiev, E.M. Yatsenko, N.K. Fomina, L.N. Mikhina, L.P. Zhavoronkov, V.V. Yuzhakov, A.G. Konoplyannikov
A.F. Tsyb Medical Radiological Research Center – branch of the National Medical Research Radiological Сentre of the Ministry
of Health of the Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249031, Russian Federation
References
Gupta P.B., Onder Т.T., Jiang G., Tao K., Kuperwasser C., Weinberg R.A., Lander E.S. Identification of selective inhibitors of cancer stem cells by high-throughput screening. Cell. 2009; 138 (4): 645–59. DOI: 10.1016/j.cell.2009.06.034
Huczynski A. Salinomycin: a new cancer drug candidate. Chem. Biol. Drug. Des. 2012; 79 (3): 235–8. DOI: 10.1111/j.1747-0285.2011.01287
Zhang Y., Zhang H., Wang X., Wang J., Zhang X, Zhang Q. The eradication of breast cancer and cancer stem cells using octreotide modifed paclitaxel active targeting micelles and salinomycin passive targeting micelles. Biomaterials. 2012; 33 (2): 679–91. DOI: 10.1016/j.biomaterials.2011.09.072
Москалева Е.Ю., Северин С.Е. Противоопухолевая активность ионофорного антибиотика салиномицина: мишень – опухолевые стволовые клетки. Молекулярная медицина. 2012; 6: 28–37. DOI: 10.29296/24999490-2018-02-02. [Moskaleva Е.Yu., Severin S.Е. Anti-tumor activity of the ionophore antibiotic salinomycin: the target – cancer stem cells. Molekulyarnaya meditsina. 2012; 6: 28–37. DOI: 10.29296/24999490-2018-02-02 (in Russian)]
Москалева Е. Ю. Кондрашева И.Г., Попова О.Н., Семочкина Ю.П., Шмаргун А.М., Северин С.Е. Цитотоксическая активность ионофорного антибиотика салиномицина и его комбинации с противоопухолевыми препаратами в отношении меланомы. Молекулярная медицина. 2013; 3: 56–61. [Moskaleva E.Yu., Kondrasheva I.G., Popova O.N., Semochkina Yu.P., Shmargun A.M., Severin S.E. Cytotoxic activity of the ionophore antibiotic salinomycin and its combination with anticancer preparations against human melanoma cells. Molekulyarnaya meditsina. 2013; 3: 56–61 (in Russian)]
Корман Д.Б., Островская Л.А, Блюхтерова Н.В., Рыкова В.А., Фомина М.М. Наночастицы золота как потенциальные радиосенсибилизирующие и цитотоксические агенты. Биофизика. 2021; 66 (6): 1229–45. DOI: 10.1134/0006350921060063. [Korman D.B., Ostrovskaya L.A, Blyuhterova N.V., Rykova V.A., Fomina M.M. Gold nanoparticles as potential radiosensitizing and cytotoxic agents. BIOPHYSICS. 2021; 66 (6): 1229–45. DOI: 10.31857/000630292106020 (in Russian)]
Пальмина Н.П., Сажина Н.Н., Богданова Н.Г., Антипова А.С., Мартиросова Е.И., Плащина И.Г., Каспаров В.В., Семёнова М.Г. Физико-химические свойства липосом, реконструированных из липидов печени и головного мозга мышей, принимавших нанолипосомальные комплексы. Биофизика. 2021; 66 (5): 925–36. DOI: 10.31857/0006302921050100 [Palmina N.P., Sazhina N.N., Bogdanova N.G., Antipova A.S., Martirosova E.I., Plashchina I.G., Kasparov V.V., Semenova M.G. The physico-chemical properties of liposomes made from lipids of the liver and brain of mice receiving nanolipid complexes. BIOPHYSICS. 2021; 66 (5): 925–36 (in Russian)]
Новикова О.Д., Набережных Г.А., Сергеев А.А. Наноструктурные биосенсоры на основе компонентов бактериальных мембран. Биофизика. 2021; 66 (4): 668–83 DOI: 10.31857/0006302921040062 [Novikova O.D., Naberezhnykh G.A., Sergeev A.A. Nanostructured biosensors based on components of bacterial membranes. BIOPHYSICS. 2021; 66 (4): 668–83 (in Russian)]
Коноплянников А.Г, Алексенский А. Е., Злотин С. Г., Смирнов Б. Б., Кальсина С.Ш., Лепехина Л. А., Семенкова И. В., Агаева Е. В., Бабоян С. Б., Рюмшина Е.А., Носаченко В. В., Коноплянников М. А. Комплексы детонационных наноалмазов с ингибиторами раковых стволовых клеток или паракринными продуктами мезенхимальных стволовых клеток как новые потенциальные лекарственные средства. Кристаллография. 2015; 60 (5): 831–6. DOI: 10.7868/0023476115050045. [Konoplyannikov A.G, Aleksenskij A.E., Zlotin S.G., Smirnov B.B., Kal'sina S.Sh., Lepehina L.A., Semenkova I.V., Agaeva E.V., Baboyan S.B., Ryumshina E.A., Nosachenko V.V., Konoplyannikov M.A. Complexes of detonation nanodiamonds with cancer stem cell inhibitors or paracrine products of mesenchymal stem cells as new potential drugs. Crystallography. 2015; 60 (5): 831–6. DOI: 10.7868/0023476115050045 (in Russian)]
Петриев В.М., Тищенко В.К., Михайловская А.А., Коноплянников А.Г. Фармакокинетика наноалмазов, меченных 188 Re, в организме мышей с экспериментальной карциномой Эрлиха. Радиация и риск. 2017; 26 (2): 62–71. DOI: 10.21870/0131-3878-2017-26-2-62-71. [Petriev V.M. Tishchenko V.K., Mikhailovskaya A.A., Konoplyannikov A.G. Pharmacokinetics of 188Re-nanodiamonds complex in mice bearing experimental Ehrlich carcinoma. Radiation & Risk. 2017; 26 (2): 62–71. DOI: 10.21870/0131-3878-2017-26-2-62-71 (in Russian)]
Коноплянников М.А., Тимошенко В.Ю., Каргина Ю.В., Юсубалиева Г.М., Кальсин В.А., Коноплянников А.Г., Баклаушев В.П., Тимашев П.С. Комплекс салиномицина с наночастицами кремния эффективно ингибирует опухолевый рост in vitro и in vivo. Гены и Клетки. 2019; 14 (3): 117. DOI: 10.23868/gc122870. [Konoplyannikov M.A., Timoshenko V.Y., Kargina Y.V., Yusubalieva G.M., Kal'sin V.A., Konoplyannikov A.G., Baklaushev V.P., Timashev P.S. The complex of salinomycin with silicium nanoparticles effectively inhibits tumor growth in vitro and in vivo. Genes & Cells. 2019; 14 (3): 117. DOI: 10.23868/gc122870 (in Russian)]