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Novel strategies for post-exposure rabies prophylaxis: the role of immunomodulatory and targeted molecular technologies in personalized medicine

Potupchik T.V., Generalov S.V., Akaeva A.V., Shablinskaya K.S

Introduction. Rabies is currently a rare but deadly viral disease transmitted through animal bites, mainly dogs. There is no standardized effective therapy for clinically manifest rabies. Patients with developed symptoms are mainly provided with palliative car…

DECREASE IN ANG AND VEGF mRNA LEVELS DURING PROGRESSIVE ANGIOGENESISOF THE LIVER VENOUS SYSTEM OF WISTAR RATS IN EXPERIMENTAL CIRRHOSIS

Introduction. Currently, understanding the molecular mechanisms of pathological angiogenesis remains a fundamental problem in hepatology. The aim of this work was to find a relationship between the level of mRNA expression of the ang, vegf genes and angiogenes…

СYTOKINES FOR ALLERGIC DISEASES

Morozova O.V., Ospelnikova Т.P.

The majority of IgE binds with Fcε mast cells receptors. Repeated contacts between allergen and specific IgE on the surface of mast cells result in Fcε-receptors cross-linking, degranulation and release of inflammation mediators such as histamine, proteases, c…

INNATE AND ADAPTIVE IMMUNITY DURING INFECTION AND IMMUNIZATION WITH INACTIVATED TICK-BORNE ENCEPHALITIS VACCINE

Morozova O.V., Isaeva E.I.

Introduction. Viral infections induce cellular and humoral immunity that provide virus elimination. Extracellular presentation of antigens of formaldehyde-inactivated vaccines causes mainly humoral immune response with risk of persistent infection. The aim of …

MicroRNA AND ITS’ TARGETS: BASICS OF BOIINFORMATIC SEARCH

Shestakov A.V., Mikhailova A.A., Saprina T.V., Onkhonova O.E.

MicroRNAs are defined as short non-coding molecules that regulate gene expression at the post-transcriptional level through complementary binding to the corresponding mRNA. This pairing leads to inhibiting of further translation by mRNA degradation, in other w…

Alterations in mRNA expression of rat astrocytes co-cultured with c6 glioma cells and impact of glioma-conditioned astrocytes on dendritic cell maturation

Chernysheva A.A., Chekhonin I.V., Sosnovtseva A.O., Cherepanov S.A., Kardashova K.Sh., Gurina O.I., Silantyev A.S., Pavlova S.A., Pavlova G.V., Savelieva T.A., Loshhenov V.B., Chekhonin V.P.

Introduction. Under the influence of glioma cells, peritumoral zone astrocytes may acquire tumorigenic phenotype, particularly due to the suppression of immunocompetent cells. The aim of the study. Investigate the changes in the gene expression profile of glio…

EXPRESSION OF GLIAL MARKERS, CYTOKINES AND MARKERS OF NEUROGENESIS IN THE MOUSE HIPPOCAMPUS DURING AGING AND IN RESPONSE TO LIPOPOLYSACCHARIDE

Sotnikov E.B., Patlay N.I., Nikolaeva A.Y., Tuchina O.P.

Introduction. Aging is associated with a decrease in the neuro- and synaptogenesis as well as hyperactivation of glial cells, which leads to changes in the morphofunctional state of the nervous tissue of the hippocampus and, as a consequence, a deterioration i…

Features of local expression of mRNA, IL-1β, IL-18, CCL2/MCP-1 in modeling pigment epithelium atrophy and retinal degeneration in the experiment on rabbits

Neroev V.V., Balatskaya N.V., Svetlova E.V., Neroeva N.V., Ryabina M.V., Karmokova A.G., Losanova O.A., Chernomorets I.Yu., Ilyukhin P.A.

Introduction. Degenerative retinal diseases are considered as the leading cause of blindness and low vision worldwide. The last studies have provided convincing evidence of the involvement of immunological factors in the development and progression of the dise…

THE LEVEL OF EXPRESSION OF PROGESTERONE, ESTRADIOL AND VITAMIN D RECEPTORS IN MONONUCLEAR CELLS OF THE PERIPHERAL BLOOD OF PATIENTS WITH SURGICAL MENOPAUSE

Kruchinina E.V., Gorenkova O.S., Glazkova A.V., Polyakova E.Yu., Kochina N.A., Krasnoshchok E.V., Zaydieva Y.Z., Kareva E.N., Serebrova S.

Introduction. Patients with surgical menopause are at risk for an osteopenic syndrome. For adequate medical correction of osteoporosis, it is necessary to clarify the mechanisms of the pathogenesis of this condition. It is known that osteoclasts, the activatio…

EXPRESSION OF FACTORS ACTIVATED BY HYPOXIA, GROWTH FACTOR VEGF, ITS RECEPTOR, CARBOHYDRASE IX IN TISSUE IN PAPILLARY CANCERs AND METASTASES

Spirina L.V., Malysheva K.S., Chizhevskaya S.U., Kondakova I.V., Kovaleva I.V.

Introduction. The molecular mechanisms of the development of tumor pathology of the thyroid gland are associated with the activation of angiogenesis and the development of hypoxia, which contributes to the proliferation, invasion, and progression of the diseas…

Prediction of the blastulation onset in one day behind embryos by the expression profile of small non-coding RNAs

Drapkina Yu.S., Timofeeva A.V., Chagovets V.V., Makarova N.P., Frolova A.M., Kalinina E.A.

Introduction. Successful embryonic development immediately after fertilization depends on the maternal messenger RNAs (mRNAs) destruction and zygotic genome transcription activation during maternal to zygotic transition (MZT). Key regulators of MZP are small n…

THE PROFILES OF LACTOBACILLLUS MICROFLORA AND LOCAL IMMUNE RESPONSE OF THE VAGINAL MUCOSA IN THE DIAGNOSIS OF VAGINAL INFECTIONS

Budilovskaya O.V., Krysanova A.A., Shipitsyna E.V., Perever- zeva N.A., Vorobyova N.E., Gerasimova E.N., Grigoryev A.N., Savi- cheva A.M.

Introduction. In vaginal infections, both microbial factor and the state of local (mucosal) immunity, determined by the immunological reactivity of the macroorganism, play an important role in the pathogenesis of an infectious process. Aim of the studying. To …

THE LEVEL OF EXPRESSION OF PROGESTERONE, ESTRADIOL AND VITAMIN D RECEPTORS IN MONONUCLEAR CELLS OF THE PERIPHERAL BLOOD OF PATIENTS WITH SURGICAL MENOPAUSE

Kruchinina E.V., Gorenkova O.S., Glazkova A.V., Polyakova E.Yu., Kochina N.A., Krasnoshchok E.V., Zaydieva Y.Z., Kareva E.N., Serebrova S.

Introduction. Patients with surgical menopause are at risk for an osteopenic syndrome. For adequate medical correction of osteoporosis, it is necessary to clarify the mechanisms of the pathogenesis of this condition. It is known that osteoclasts, the activatio…

THE ROLE OF TRANSCRIPTION AND GROWTH FACTORS IN THE DEVELOPMENT OF THE TUMOR PATHOLOGY OF THE PROSTATE GLAND

Spirina L.V., Kovaleva I.V., Fedotov D.A., Gorbunov A.K., Usynin E.A., Slonimskaya E.M., Kondakova I.V., Dyakov D.A.

Introduction. The molecular mechanisms of the development of the prostate tumor pathology are associated with the activation of both androgen-dependent and androgen-independent signaling pathways, accompanied by the development of chronic inflammation and hypo…

THE ROLE OF STEROID ER, AR RECEPTORS, BRN-3Α AND TRIM16 NUCLEAR TRANSCRIPTION FACTORS IN THE DEVELOPMENT OF KIDNEY CANCER

Spirina L.V., Kondakova I.V., Yurmazov Z.A., Usynin Е.А., Slonimskaya Е.М.

Introduction. The study of molecular mechanisms of the development of kidney cancer is one of the actual problems in oncology. There is conflicting information about the importance of steroid hormone receptors in the kidney cancer. The aim of the study was to …

PROGNOSTIC SIGNIFICANCE EXPRESSION OF MRNA TOP1, TOP2Α, TUBΒ3, TYMS, ABCC5 AND GSTP1 GENES IN PATIENTS WITH NON-SMALL CELL LUNG CANCER

Deryusheva I.V., Tsyganov M.M., Rodionov E.O., Efteev L.A., Ibragimova M.K., Miller S.V., Tuzikov S.A., Litviakov N.V.

Introduction. Despite the continuous improvement of the surgical method of treatment of patients with non-small cell lung cancer (NSCLC), there is no noticeable tendency to improve survival data. This 5-year index does not exceed 15–25%. In this regard, it is …

THE LEVEL OF NITRIC OXIDE METABOLITES AND NOS2 GENE TRANSCRIPTS IN RHEUMATOID ARTHRITIS

Topchieva L.V., Balan O.V., Malysheva I.E., Barysheva O.Yu., Marusenko I.M., Vaskova O.A.

The aim of this work was to study the effect of methotrexate, glucocorticoids and nonsteroidal anti-inflammatory drugs on the plasma levels of nitric oxide metabolites, as well as the level of NOS2 gene transcripts in peripheral blood leukocytes (PBL) in rheum…

THE ROLE OF P53 AND NOXA IN THE PATHOGENESIS OF EARLY MISCARRIAGES

Lebedeva O.P., Zhukova I.O., Ivashova O.N., Pakhomov S.P., Churnosov M.I.

Introduction. Missed and spontaneous abortions are one of the most important causes of the reproductive loss. The aim of the study: to study specialties of mRNA expression of p53 and proteins of its signaling pathway in the decidual tissue at 6–10 weeks of ges…

OVEREXPRESSION OF APOPTOTIC ENDONUCLEASE ENDOG INHIBITS TELOMERASE ACTIVITY IN HUMAN CD4+ T CELLS

Vasina D.А., Zhdanov D.D., Orlova E.V., Orlova V.S., Pokrovskaya M.V., Aleksandrova S.S., Sokolov N.N.

Introduction. Alternative splicing of telomerase catalytic subunit hTERT (human Telomerase Reverse Transcriptase) regulates the telomerase activity. The increased expression of non-active spliced hTERT results in inhibition of telomerase. However, very little …

ANALYSIS OF THE INTERFERONS OF I, II AND III TYPES, IL23 AND ANTIVIRAL PROTEIN MXA FOR MULTIPLE SCLEROSIS

Ospelnikova T.P., Morozova O.V., Isaeva E.I., Lizhdvoy V.Yu., Kolodyaznaya L.V., Andreeva S.A., Kotov S.V., Еrshov F.I.

Introduction. A lot of factors of innate and specific immunity are involved in development of the autoimmune diseases – multiple sclerosis (MS) that cause the damages of myelin envelopes of nerve fibres with irreversible neurological consequences. To decrease …