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Quantitative indicators of TREC and KREC as predictors of the severity of acute respiratory infections in children

Ligskaya E.V., Eremeeva A.V., Kudlay D.A., Satyshev O.V., Gordukova M.A., Korsunsky A.A.

Аmphocyte production and indirectly reflect the state of the adaptive immune system. Their concentration in dried blood spots can be determined by quantitative polymerase chain reaction (qPCR). Currently, these markers are used for neonatal screening of primar…

The effect of a high-fat diet and phytocomposition based on B. vulgaris, C. bergamia, D. villosa and L. meyenii on the expression of genes for carbohydrate and lipid metabolism enzymes in the liver of rats

Yankovskaya S.V., Mosalev K.I., Deulin I.Y., Palchikova N.A., Selyatitskaya V.G.

Introduction. The liver serves as a buffer for the accumulation and utilization of excess intake of nutritional fats, the intake of plant compounds can improve metabolic processes in the liver, but the molecular mechanisms of this phenomenon have been little s…

Molecular genetic features of iron deficiency anemia phenotypes

Bakirova A.E., Parcernyak A.S., Kudlay D.A., Chumakova A.K., Abdeeva G.R.

Relevance. Iron deficiency anemia is a disease present all over the world and maintaining a high incidence rate of both general and primary morbidity despite various options for correction and prevention of this pathology. Molecular genotyping opens up new pos…

The effect of polymorphism of the NQO1 gene on the content of ubiquinone in blood plasma and the state of functional activity of mitochondria in patients with chronic heart failure

Lobanova O.A., Gaikovaya L.B., Sirotkina O.V., Kukharchik G.A., Ermakov A.I., Zagorodnikova K.A.

Introduction. Chronic heart failure is a multifactorial disease, an important role in the pathogenesis of which is played by disorders of the functions of mitochondria and energy metabolism in cardiomyocytes, as well as a significant contribution is made by ge…

TELOMERE LENGTH IN RHESUS MACAQUES WITH VOLUNTARY CHRONIC ETHANOL CONSUMPTION

Timina M.F., Kirgintsev R.M., Pavlova L.E., Agumava A.A., Panchenko A.V.

Introduction. Alcohol abuse is associated with telomere shortening. There is no convincing evidence of a “safe” level of alcohol consumption in this regard. Long-term studies in rodents are not feasible, and clinical trials with the administration of alcohol t…

Genetic predictors of the risk of developing complications in the immediate postoperative period following combat trauma

Kishenya M.S., Sobolev D.V., Anchikova E.V., Visyagin A.V.

Introduction. Paratraumatic eczema (PTE), being a type of microbial eczema, occurs after surgical interventions, chronic wound healing, accompanied by pronounced inflammatory changes with hyperemia, edema, exudation, soreness and itching. Genetic polymorphisms…

INFLUENCE OF FIBROBLAST GROWTH FACTOR-2 ON THE ACTIVATION OF MOUSE HIPPOCAMPAL MICROGLIA IN A LIPOPOLYSACCHARIDE-INDUCED NEUROINFLAMMATION MODEL IN VITRO

Shults M.R., Shults A.S., Tuchina O.P.

Introduction. Activation of the pro-inflammatory phenotype of microglia may be one of the causes of chronic neuroinflammation and, as a result, can lead to pathological conditions of the brain. The use of neurotrophic factors, such as FGF2, may become one of t…

Molecular biological analysis of clinical and pathogenetic features of the formation of postoperative complications in combat trauma

Sobolev D.V., Kishenya M.S., Visyagin A.V., Anchikov E.V.

Introduction. Pathological scars (PS) occur after traumatic injuries, surgical interventions, burns, etc. lead to functional disorders, cosmetic defects, and also significantly affect the quality of life. The results of studies to determine the genetic determi…

DECREASE IN ANG AND VEGF mRNA LEVELS DURING PROGRESSIVE ANGIOGENESISOF THE LIVER VENOUS SYSTEM OF WISTAR RATS IN EXPERIMENTAL CIRRHOSIS

Introduction. Currently, understanding the molecular mechanisms of pathological angiogenesis remains a fundamental problem in hepatology. The aim of this work was to find a relationship between the level of mRNA expression of the ang, vegf genes and angiogenes…

AED PEPTIDE ACTIVATES GENE EXPRESSION AND DIFFERENTIATION PROTEINS SYNTHESISOF HUMAN SKIN FIBROBLASTS DURING REPLICATIVE AGING

Gutop E.O., Linkova N.S., Fridman N.V., Kozhevnikova E.O.,Polyakova V.O., Khavinson V.Kh.

The aim of this work is to study the effect of the AED peptide on fibroblast differentiation of human skin when aging in vitro. Methods. The study was conducted on DF-1 cell line (human dermal fibroblasts (mesenchymal stem cells)) isolated from the skin of the…

THE ASSOCIATION OF rs34643859 GENE KCNS1, rs12804550 GENE SCN4B, rs4514993 GENE SCN11A WITH THE SUDDEN CARDIAC DEATH

Ivanova А.А., Melnikova E.S., Gurazheva A.A., Malutina S.K., Rodina I.A., Khamovich O.V., Novoselov V.P., Maksimov V.N.

Introduction. Single nucleotide polymorphisms rs34643859 of the KCNS1 gene, rs12804550 of the SCN4B gene, and rs4514993 of the SCN11A gene were revealed in the analysis of the results of whole exome sequencing of a group of young man died by sudden cardiac dea…

THE PROFILES OF LACTOBACILLLUS MICROFLORA AND LOCAL IMMUNE RESPONSE OF THE VAGINAL MUCOSA IN THE DIAGNOSIS OF VAGINAL INFECTIONS

Budilovskaya O.V., Krysanova A.A., Shipitsyna E.V., Perever- zeva N.A., Vorobyova N.E., Gerasimova E.N., Grigoryev A.N., Savi- cheva A.M.

Introduction. In vaginal infections, both microbial factor and the state of local (mucosal) immunity, determined by the immunological reactivity of the macroorganism, play an important role in the pathogenesis of an infectious process. Aim of the studying. To …

REPROGRAMMING OF THE GENOME IN ADAPTIVE RESPONSES OF THE CELL

Khabarov V.N., Boykov P.J., Ivanov P.L., Moskovtsev A.A.

Introduction. New directions of the modern cell biology are the development of methods for reprogramming stem cells into differentiated ones and reverse reprogramming of differentiated cells into stem cells. Transient processes in cells from the one stable sta…

POLYMORPHISM AND SYNERGISM OF THE HEMOSTASIS SYSTEM GENES IN PATHOGENESIS OF INFECTIVE ENDOCARDITIS

Maltseva N.V., Laputenko T.A., Smirnova A.Sh., Kan S.L.

Introduction. The genetically determined tendency of patients to thrombosis may be one of the etiopathophysiological factors of the infective endocarditis (IE). Aim. А search for variants of genes and their combinations in the hemostatic system, the carriage o…

THE EXPRESSIVE METHOD OF DNA ISOLATION FROM FROZEN BLOOD

Maltseva N.V., Smirnova A.Sh., Kolbasko A.V., Onishchenko A.L., Rublevskaya A.S.

Introduction. Mass genotyping, aimed both at identifying of genetic characteristics of populations and the creation of genomic libraries, scientific research, was implemented on large samples, required the accumulation and/or conservation of samples of the bio…

THE NEW MOLECULAR GENETIC MARKERS OF THE SUDDEN CARDIAC DEATH IDENTIFIED BY NEXT-GENERATION SEQUENCING

Ivanova А.А., Maksimov V.N., Malutina S.K., Kolesnik K.N., Aleshkina A.V., Savchenko S.V., Novoselov V.P., Voevoda М.I.

The aim of the study is an investigation of the association between mutations A1744S of MYH7, 9928_9929insE of TTN, T171M of CACNA1C, A189T of JPH2, S434Y of VCL, H4552R of RYR2, Y1495X of SCN5A and the sudden cardiac death (SCD). Investigating mutations were …

DETERMINATION OF TELOMERASE ACTIVITY BY REAL TIME DOUBLE TELOMERIC REPEAT AMPLIFICATION PROTOCOL

Glukhov A.I., Nalobin D.S., Gordeev S.A., Kalabushev S.N.

Introduction.The telomerase activity is a universal marker for 85% of malignant tumors. This allows us to develop modern highly sensitive and highly specific kits for the diagnosis of most malignant tumors, including early stages of the disease. Existing metho…

THE PREVALENCE OF CYP2C19*2, *3 POLYMORPHISMS AMONG RUSSIANS AND YAKUTS WITH PEPTIC ULCER DISEASE, LIVING IN SAKHA REPUBLIC (YAKUTIA)

Temirbulatov I.I., Ivashchenko D.V., Rudykh Z.A., Popova N.V., Tayurskaya K.S., Chertovskikh Ya.V., Sychev D.A.

Introduction. Proton pump inhibitors (PPI) are widely spread as antisecretory drugs for a treatment of acid diseases, such as the gastric ulcer. Most of the proton pump inhibitors are inactivated in the liver by cytochromes P450 2C19 (CYP2C19) to the form of i…

GENETIC POLYMORPHISM OF F2, F5 AND VKORC1 IN POPULATIONS OF BURYATS, TELEUTS AND RUSSIANS OF THE EASTERN SIBERIA

Tabikhanova L.E., Osipova L.P., Churkina T.V., Voronina E.N., Filipenko M.L.

Introduction. The study of the prevalence of genetic risk factors for thrombosis-associated diseases in the indigenous population of Siberia is an important issue in current medicine. The aim of the study. The aim of this work is to study the allele frequencie…

THE ROLE OF P53 AND NOXA IN THE PATHOGENESIS OF EARLY MISCARRIAGES

Lebedeva O.P., Zhukova I.O., Ivashova O.N., Pakhomov S.P., Churnosov M.I.

Introduction. Missed and spontaneous abortions are one of the most important causes of the reproductive loss. The aim of the study: to study specialties of mRNA expression of p53 and proteins of its signaling pathway in the decidual tissue at 6–10 weeks of ges…