Abstract
Introduction. Alcohol abuse is associated with telomere shortening. There is no convincing evidence of a “safe” level of alcohol consumption in this regard. Long-term studies in rodents are not feasible, and clinical trials with the administration of alcohol to healthy individuals is not ethically acceptable. An approach based on a relevant model of voluntary alcohol consumption in monkeys under controlled conditions is a significant alternative.
The aim of the study. To estimate the length of telomeres at long-term ethanol consumption by male rhesus macaques in under free choice with water
Methods. The study was performed on fourteen mature male Rhesus macaques of groups with low (median 0.62 g/kg/day) and high (median 2.71 g/kg/day) ethanol consumption as 4% (v./v.) solution with condition of all-day access and free choice with drinking water. The duration of consumption was 920 days. The relative length of telomeres was determined by quantitative PCR according to Cawthon (2002) in blood leukocytes.
Results. The relative average telomere length in the high-consumption group was 1.53±0.57 before the presentation of ethanol in the adaptation period (-32 day of the study), and at the consumption stage it was on 717 day 2.13±0.19 and on 917 day 4.61±0.7. In the low-consumption group, the average relative telomere length constituted 1.42±0.22, 1.55±0.15 and 3.3±0.47, respectively. The absolute count of leukocytes did not change significantly during the study. However, changes in the differential white cells count were revealed representing development of relative monocytosis by 917 day in both groups.
Conclusion. The data obtained do not confirm the association of long-term alcohol consumption in moderate doses with telomere length. The completed study has limitations related to the lack of control without consumption and evaluation in one sex.
Authors and affiliations
M.F. Timina, R.M. Kirgintsev, L.E. Pavlova, A.A. Agumava, A.V. Panchenko
Kurchatov Complex of Medical Primatology of FSBI “National Research Center “Kurchatov Institute”,
pl. Akademika Kurchatova, 1, Moscow, 123182, Russian Federation
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