Abstract
Introduction. There were examined 195 patients with chronic сerebrovasсular pathology and 149 healthy residents of the Zabaykalsky region. The aim of the study. Detection of genetic polymorphism in cytokines IL4-589С>T, IL10-1082G>A, IL10-819C>T, IL10-592C>A, and also the determination of the amount of IL1β, RA IL1, IL4, IL10, TNFα in blood plasma. Methods. The polymerase chain reaction for DNA of leukocytes of blood, the enzyme immunoassay of blood plasma samples. Results. In patients with cerebral chronic ischemia, also as well as in healthy examinees, carriers of genotypes of IL4-589-TT the decrease in concentration of IL10 in group of control to 3,47±0,00 pkg/ml, in chronic cerebral ischemia (CC) group to 6,81±2,23 pkg/ml was revealed, in owners of IL10-1082AA the amount of IL10 decreased in group of control to 3,35±1,41 pkg/ml and in CCI group – to 7,37±1,03 pkg/ml respectively (р<0,05), the increase of concentration of IL1 (р<0,05) to 0,46±0,00 pkg/ml in group of control and 0,06±0,00 pkg/ml in CCIB group is noted at IL4-589-TT carriers, in owners IL10-1082AA in CCI group: 0,49±0,00 pkg/ml , in group of control to 0,91±0,00 pkg/ml (р<0,05; р<0,001), in comparison with level of same interleukines in owners of IL4-589CC, IL10-1082GG genotypes. The rise of quantity of TNFα was also noted in a carriage of IL10-819TT, IL4-589-TT genotypes in comparison with level of same interleukines in owners of IL10-819CC, IL4-589-CC. Conclusion. From the obtained data it is possible to assume the involvement of studied genetic factors in appearance of imbalance in cytokines with shift towards pro-inflammatory markers in patients with chronic сerebrovasсular pathology.
Authors and affiliations
A.S. Knyazeva, N.N. Strambovskaya, P.P. Tereshkov Chita State Medical Academy, Gorkogo str., 39A, Chita, 672090, Russian Federation
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