Abstract
Introduction. The assessment of placental dysfunction in clinical practice remains to be subject of debates, the search of sustaining morphological markers continues. The aim of the study was to find the correlation between the expression of vimentin and placental lactogen with Doppler intraplacental vessels parameters in cases with physiological pregnancy (PP) and placental insufficiency (PI). Materials and methods. A total of 10 singleton pregnant women including 5 PP and 5 PI cases were observed in the III trimester: Fetometry, Doppler in the main arteries of a functional system of mother-placenta-fetus intraplacental vessels under a volume image reconstruction (Vocal, Voluson-730 expert, GE), laser scanning confocal microscope villous tree of placentas obtained after term delivery, the identification of expression of vimentin and placental lactogen (PL) in the central and edge portions of the placenta were performed. Results. The expression of PL area in PI cases was found to be lower than in normal pregnancy in the central parts of placenta for 51,5%, in the edge – for 35,6% (p<0,05). The brightness of the PL expression in PI group was higher than in normal pregnancy central placenta parts for 27,1% in the edges – 19,5%. In the central and edge parts of the placenta the average expression area of vimentin in PI cases was 34,9% and 49,0% less, and the brightness was 19,7% and 21,5% higher respectively, than in normal pregnancy (p<0,05). In PI placentas an inverse correlation between the flow index (FI) and an area of expression of vimentin in the central portions of the placenta was revealed (r=-0,51; p<0,05). Conclusion. The established correlation between the FI and the area of vimentin expression in the placenta makes possible to develop morphological and functional parallels that allow objectively evaluate the degree of morphological disorders in PI in clinical practice.
Keywords
Authors and affiliations
Dusembinova Sh.D., Drobintseva A.O., Sosnina А.К., Zakurina A.N. , Polyakova V.O., Pavlova N.G., Kvetnoy I.M.
References
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