FREE RADICAL OXIDATION OF PROTEINS AND LIPIDS IN ADIPOCYTES UNDER CONDITIONS OF OXIDATIVE STRESS

Introduction. According to the current understanding, oxidative stress plays a key role in the mechanisms of diabetes mellitus of types 1
and 2 emergence and development of its complications; formation of oxidative stress is accompanied by defective uptake of glucose by adipose
tissue, reduction of insulin secretion by pancreatic B-cells, adipokines production dysregulation, progression of insulin resistance, followed
by the development of metabolic syndrome.
The aim of the study. Investigate the status of oxidative modification of proteins and lipids in adipocyte epididymal adipose tissue in
oxidative stress.
Methods. Adipocytes were isolated from the epididymal adipose tissue of rats using collagenase. The fat cells were incubated with alloxan
in a concentration of 0,5–10,0 mmol/l. The content of active oxygen forms, lipid hydroperoxides, products which react with thiobarbituric
acid, carbonyl derivatives of proteins, bityrosine and oxidized tryptophan in adipocytes was determined.
Results. During experiments in vitro, the oxidative stress developed in adipocytes of epididymal adipose tissue when adding alloxan
(0,5–10 mМ) to cell incubation medium. It was accompanied by concentration increase of oxygen active forms, products, reacting with
thiobarbituric acid, hydroperoxides lipids, carbonyl derived proteins, bityrosine and oxidized tryptophan.
Conclusions. In adipocyte increased generation of reactive oxygen and an increase in the intensity of oxidative modification of
macromolecules leads to changes in their physical and chemical condition and infringement of functions that ultimately exacerbates diabetes
and contributes to the development of complications.

E.V. Shakhristova, E.A. Stepovaya, V.V. Ivanov, O.L. Nosareva, A.N. Dzuman, N.V. Ryazantseva, V.V. Novitskiy
Siberian State Medical University, Russian Federation, 634050, Tomsk, Moscow trakt, 2