Abstract
Introduction. Early diagnostics of such oncological disease as cutaneous malignant melanoma is strictly important for the decrease of mortality. It is known that microRNA expression in melanoma cells and in bening melanocytic nevi is different, that can be used for improvement of early melanoma diagnostics. The aim of the study. The evaluation of microRNA expression profiles in melanoma and bening melanocytic nevi on the base of microarray; the identification of pathways and target genes for altered microRNA by means of bioinformatics analysis to expanse the view of the melanocytic tumors pathogenesis and to reveal new diagnostic markers. Material and methods. The study included biopsies of patients with melanoma and bening melanocytic nevi. The human melanoma cell line BRO was chosen to investigate the influence of miR-4286 inhibition on target genes expression. The microRNA expression profiles were estimated with a microarray and followed by bioinformatics analysis. MicroRNA target genes expression was assessed by real-time PCR. Results. The 16.15-fold increase of miR-4306 expression level (pFDR=0,036), the 2.11-fold decrease of miR-6853-3p expression level (pFDR=0,036) were revealed in melanoma compared with bening melanocytic nevi. The pathways, proteins, and molecular functions of the detected target genes of the microRNA are associated, mostly, with cell proliferation, motility, and migration. The miR-4286 inhibition in melanoma BRO cells results in the decrease of miR-4286 target genes CCND1 and PLXNA2 expression. Conclusion. The microRNAs expression levels alteration can be supposed to associated with the stages of cell malignant transformation. MicroRNA profiling can be used both the improved comprehension of functional aspects of cancer development and the malignant tumor diagnostics.
Authors and affiliations
S.V. Tsyrenzhapova(1), R.N. Belonogov(1), E.Y. Sergeeva(1, 2), T.G. Ruksha(1) 1-Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University; Partizana Zheleznyaka str., 1, Krasnoyarsk, 660022, Russian Federation; 2-Siberian federal university, Svobodny pr., 79, Krasnoyarsk, 660041, Russian Federation E-mail: office@sfu-kras.ru
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