Abstract
Introduction. Type 1 diabetes mellitus is a chronic autoimmune disease associated with severe complications of organs and systems, including complications of the course of dental diseases.
Material and methods. Results of 55 patients with type 1 DM became the material of laboratory-diagnostic studies. Patients with type 1 diabetes were divided into 2 groups depending on blood glucose levels: Group 1 (n=28) – patients with glucose levels ≥7 mmol/l; Group 2 (n=27) – patients with glucose levels ≤7 mmol/l; Control group – 28 patients. Periodontal tissues were evaluated by indices: PMA, SBI. OHI-S index reflected the state of oral hygiene. Mucosal immunity of oral fluid was studied using ELISA analysis of pro- and anti-inflammatory cytokines IL-8, IL-10, IL-1β, MCP-1, TNF-α, VEGF-А.
Results. The content in unstimulated oral fluid of IL-1β in patients with type 1 DM was 84.1 (95–74.6) pg/mL in the group with glucose ≤ 7 mmol/L, 80.1 (115.4–64.9) pg/mL in the group with glucose ≥7 mmol/L, 23.1 (28.2–18.6) pg/mL in the comparison group, VEGF-A was 1340 (1545–967.5) pg/mL, 1170 (1517.5–954.8) pg/mL in group 2, and 1655 (1860–1370.5) pg/mL in group 3. TNF-α was higher in group 1 – 9.8 (11.4–7.5) pg/mL than in group 2 – 8.3 (10.3–6.6) pg/mL and in group 3 – 1 (1.1–0.9) pg/mL (p < 0.05).
Conclusion. ROC curve analysis showed that these biomarkers have better sensitivity and specificity. The increase of IL-8, TNF-α, IL-1β content in mixed saliva up to 285.5 (339.8–214.5) pg/ml, 9.8 (11.4–7.5), 84.1 (95–74.6) pg/ml, respectively, was characterized by high medians of OHI-s, PMA and SBI indices, associated with high activity of inflammatory-destructive changes in periodontal tissues and impaired metabolic control of glucose level.
Authors and affiliations
M.M. Shcherbakova(1), N.S. Мorozova(1), A.F. Allakhverdiyev ogly(1), I.U. Lizunova(1), A.A. Saushkina(1), N.B. Zaharova(2)
1-Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Trubetskaya str., 8, build. 2, Moscow, 119991, Russian Federation;
2-Federal State Budgetary Educational Institution of Higher Education “Saratov State Medical University named after V.I.Razumovsky”, Healthcare Ministry of the Russian Federation, Bol'shaya Kazach'ya str., 112, Saratov, 410012, Russian Federation
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