Abstract
Introduction. Ovarian cancer is characterized by high mortality. Despite available diagnostic and therapeutic methods, patient survival remains unsatisfactory, especially for high-grade tumors, necessitating the exploration of new molecular targets. Heat shock proteins Hsp27 and Hsp90, which regulate cellular stress and survival, are considered key factors in tumor progression.
Objective. To conduct a comparative assessment of the immunohistochemical detection of Hsp27 and Hsp90 in high- and low-grade serous ovarian carcinomas with separate evaluation of the nuclear and cytoplasmic localization of the reaction.
Material and methods. The prospective study (NCT04817501) included 58 patients with newly diagnosed ovarian cancer. Of these, serous cancer (FIGO stages I-IIIC) was verified in 49 patients: 36 HG cases and 13 LG cases. Paraffin-embedded tumor tissue blocks obtained before treatment were studied. The study material consisted of paraffin blocks from biopsy and surgical tumor specimens. The proportions of Hsp27- and Hsp90-positive cells in the tumor and stromal components were assessed by immunohistochemistry followed by digital analysis of scanned images (Aperio AT2, Leica; QuPath software).
Results. Significant differences were revealed in the tumor component between the HG and LG groups. The proportion of Hsp27-positive tumor cells with cytoplasmic and nuclear localization of the immunohistochemical reaction was significantly higher in the HG group than in the LG group (p=0.02). No significant differences in Hsp90 were found between the groups in either the stromal or the tumor component.
Conclusion. Differences in the immunohistochemical detection of Hsp27 and Hsp90 in the tumor and stromal components of ovarian carcinoma, as well as a significant increase in the proportion of Hsp27-positive tumor cells in HGSC compared with LGSC, indicate the potential diagnostic and prognostic value of Hsp27. Furthermore, the obtained results indicate the potential of chaperone-targeted therapy as a promising direction that requires further research.
Keywords
Authors and affiliations
https://elibrary.ru/yhcncy
E.V. Kaigorodova(1, 2), O.I. Kovalеv2, M.Y. Grishchenko2, 3, E.R. Tulendinov2
1-Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Kooperativny Str., 5, Tomsk,
634009, Russian Federation; 2-Federal State Budgetary Educational Institution of Higher Education “Siberian State Medical University”
of the Ministry of Health of the Russian Federation, Moskovskiy trakt, 2, Tomsk, 634050, Russian Federation; 3State Regional
Autonomous Healthcare Institution Tomsk Regional Oncology Center, Lenin Avenue, 115, Tomsk, 634050, Russian Federation
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